Japanese Food Safety Notification (Shoku-An) No.1003001* (October 3, 2006, Ministry of Health, Labour and Welfare (MHLW)) added the “Multi-residue Method for Agricultural Chemicals by LC-MS (Animal and Fishery Products)” to Shoku-An No.0124001 (January 24, 2005) “Analytical Methods for Residual Compositional Substances of Agricultural Chemicals, Feed Additives and Veterinary Drugs in Food, Chapter II Multiresidue Method”. In both Multi-residue Method for Agricultural Chemicals by GC-MS or by LC-MS (Animal and Fishery Products), gel permeation chromatography (GPC clean-up) is employed during the purification process of test solution preparation. The method states using a GPC clean-up column of a styrene–divinylbenzene copolymer column (20 mm I.D. x 300 mm L) connected to a guard column of the same material (20 mm I.D. x 100 mm L), or equivalent products. The fractionation indicator pesticides specified are acrinathrin and tricyclazole, although the fractionation range varies depending on the type of sample. In this application, GPC clean-up column CLNpak EV-2000 AC and its guard column CLNpak EV-G AC were used for the analysis of acrinathrin and tricyclazole in compliance with the Shoku-An No.1003001.

*The version at the time of the application acquisition.

Sample: 5 mL
5 mg/L each in eluent

1. Acrinathrin
2. Tricyclazole