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Simultaneous LC/MS/MS Analysis of Mycotoxins (C18U 2B)

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The C18U series column is a silica-based reversed phase column especially designed for UHPLC use. The use of sub-2-micron organic silica hybrid particles provides superior separation efficiency and mechanical strength.  
In this application, mycotoxins, such as aflatoxins, ochratoxin, and trichothecenes, that are often found in foods and feed were analyzed simultaneously using an LC/MS/MS method with C18U 2B. By using the described method, all mycotoxins studied here were analyzed within 5 minutes.


Sample : 10 μL


Column       : Shodex C18U 2B (2.0 mm I.D. x 50 mm)
Eluent       : (A); 10 mM CH3COONH4 aq. (B); CH3CN
               Gradident: (B %) 0 to 90 % (0 to 5 min), 90 % (5 to 7 min), 
               0 % (7.01 min), 0 % (7.01 to 10 min)
Flow rate    : 0.4 mL/min
Detector     : ESI-MS/MS (MRM)
Column temp. : 40 °C

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