Aflatoxins (C18M 4E)

Aflatoxins were analyzed with Silica C18M 4E column after the sample pretreatment.

Sample : 5μg/L each, 20μL

	(TFA method)		(PR method)
1.	Derived Aflatoxin G₁	1.	Aflatoxin G₂
2.	Derived Aflatoxin B₁	2.	Aflatoxin G₁
3.	Aflatoxin G₂	3.	Aflatoxin B₂
4.	Aflatoxin B₂	4.	Aflatoxin B₁

(TFA method)                                  (PR method)
Column       : Shodex Silica C18M 4E          Column       : Shodex Silica C18M 4E 
               (4.6mmI.D. x 250mm)                           (4.6mmI.D. x 250mm)
Eluent       : CH₃CN/CH₃OH/H₂O=10/30/60        Eluent       : CH₃OH/H₂O=40/60
Flow rate    : 1.0mL/min                      Flow rate    : 0.7mL/min
Detector     : Fluorescence                   Detector     : Fluorescence
               (Ex.365nm, Em.450nm)                          (Ex.365nm, Em.450nm)
Column temp. : 40°C                           Column temp. : 40°C