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Aflatoxins (C18M 4E)

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Aflatoxins were analyzed with Silica C18M 4E column after the sample pretreatment.


Sample : 5μg/L each, 20μL

 

 (TFA method) (PR method)
1. Derived Aflatoxin G1 1. Aflatoxin G2
2. Derived Aflatoxin B1 2. Aflatoxin G1
3. Aflatoxin G2 3. Aflatoxin B2
4. Aflatoxin B2 4. Aflatoxin B1
(TFA method)                                  (PR method)
Column       : Shodex Silica C18M 4E          Column       : Shodex Silica C18M 4E 
               (4.6mmI.D. x 250mm)                           (4.6mmI.D. x 250mm)
Eluent       : CH3CN/CH3OH/H2O=10/30/60        Eluent       : CH3OH/H2O=40/60
Flow rate    : 1.0mL/min                      Flow rate    : 0.7mL/min
Detector     : Fluorescence                   Detector     : Fluorescence
               (Ex.365nm, Em.450nm)                          (Ex.365nm, Em.450nm)
Column temp. : 40°C                           Column temp. : 40°C

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