Aflatoxins are a type of mycotoxin. Under the Food Sanitation Act, the sum of aflatoxins B₁, B₂, G₁, and G₂ is referred to as “total aflatoxins”. Japanese Food Safety Notification No. 0816-1* (August 16, 2011, Ministry of Health, Labour and Welfare (MHLW)) includes “Test Method for Total Aflatoxins”, which specifies the use of an octadecylsilyl silica gel column for quantifying total aflatoxins. In this application, we introduce the analysis of aflatoxins using a fluorescence detector in compliance with this method. Aflatoxin B₁ and G₁ exhibit strong fluorescence after derivatization. Derivatization can be achieved by TFA method (trifluoroacetic acid-induced fluorescence derivatization), as well as by PR method (UV irradiation-induced fluorescence derivatization) or KC method (electrochemical bromination-induced derivatization). While the TFA method is a pre-column derivatization technique, the PR and KC methods are post-column techniques, resulting in different elution orders of aflatoxins.

*The version at the time of the application acquisition.

Sample: 20 µL each
5 µg/L each of Aflatoxins

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