Aflatoxins were analyzed with Silica C18M 4E column after the sample pretreatment.

Sample: 20 μL
5μg/L each

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No. TFA method PR method
1 Derived Aflatoxin G1 Aflatoxin G2
2 Derived Aflatoxin B1 Aflatoxin G1
3 Aflatoxin G2 Aflatoxin B2
4 Aflatoxin B2 Aflatoxin B1
For TFA method
Column
Shodex Silica C18M 4E 4.6 mm I.D. x 250 mm)
Eluent
CH3CN/CH3OH/H2O=10/30/60
Flow rate
1.0 mL/min
Detector
Fluorescence (Ex.365 nm, Em.450 nm)
Column temp.
40 ℃

 

For PR method
Column
Shodex Silica C18M 4E (4.6 mm I.D. x 250 mm)
Eluent
CH3OH/H2O=40/60
Flow rate
0.7 mL/min
Detector
Fluorescence (Ex.365 nm, Em.450 nm)
Column temp.
40 ℃

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