Separation of Substances Produced During Utilization of Biomass (VN-50 4D)

In recent years, research to utilize biomass as an energy resource has been actively conducted. The various substances such as glycerin and dihydroxyacetone are produced in the reaction process from biomass until obtaining the final product. In this application, the substances produced during biomass utilization wereseparated using HILICpak VN-50 4D, a polymer-based HILIC column.

Sample: 10 μL
(in H2O/CH3CN=20/80)

  1. 1.Dihydroxyacetone 10 mg/mL
  2. 2.Glycerin 1 mg/mL
  3. 3.Glyceraldehyde 0.025 mg/mL
  4. 4.Glyceric acid 0.025 mg/mL
  5. 5.Glycolic acid 10 mg/mL
Column
：Shodex HILICpak VN-50 4D (4.6 mm I.D. x 150 mm)
Eluent
：(A); 0.1 %HCOOH, (B); CH3CN
Linear gradient; (B %) 95 % (0 to 2 min), 95 to 20 % (2 to 7 min), 20 % (7 to 13 min)
Flow rate
：0.6 mL/min
Detector
：ELSD
Column temp.
：60 ℃

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