In order to separate hydrophilic compounds like amides, water or 100 % buffer solution is used as the eluent, however, such eluent usually shorten the column life of ODS columns. Since the packing material of RSpak DE-413 is chemically stable and has long column life. The stable analysis results can be obtained using DE-413 even when 100 % aqueous solution is used as the eluent.


Sample : 10 μL
1. Acetamide 100 mg/L
2. Propionamide 100 mg/L
3. Acrylamide 10 mg/L
4. n-Butylamide 100 mg/L
5. Methacrylamide 10 mg/L

Column       : Shodex RSpak DE-413 (4.6 mm I.D. x 150 mm)
Eluent       : 10 mM H3PO4 aq.
Flow rate    : 1.0 mL/min
Detector     : UV (210 nm)
Column temp. : 50 °C