Hydrogen peroxide is used as a sanitizer and a disinfectant in the food industry. Here, hydrogen peroxide was analyzed using a SUGAR KS-801 (a column for GFC separation) and an electrochemical detector (ECD).
(A) shows the detection of hyrogen peroxide alone. The peak of hydrogen peroxide can be seen at 21.2 minutes. The small peak at 16.8 minutes is considered to be an impurity in hydrogen peroxide.
(B) shows the chromatogram of simultaneous analysis of ascorbic acid and hydrogen peroxide.
(C) shows the autoxidation of ascorbic acid. The quantitaion was done using HPLC detection. (B) is the chromatogram at 3 hours in (C).
Sample :
Hydrogen peroxide
Ascorbic acid
Column : Shodex SUGAR KS-801 (8.0 mm I.D. x 300 mm) Eluent : H2O Reagent : 10 mM Na2SO4 + 10 μM EDTA-2Na aq. Flow rate : (Eluent); 0.6 mL/min, (Reagent); 0.15 mL/min Detector : ECD (Electrode; Pt, 500 mV vs Ag/AgCl) Column temp. : 40 °C
Takahashi et al., Analytycal Sciences, 15 (1999) 481
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Applications
- Mild Hydrolysates of Allosamidin (ES-502C 7C)
- 5-(Hydroxymethyl)Furfural in Fruit Juice (ODP-50 6D)
- Cyanate and Carbamyl Phosphate (ES-502N 7C)
- Chelating Reagents (1) (I-524A)
- Chelating Reagents (2) (NI-424)
- Analysis of Chelating Agents (ODP-50 4D)
- Hydantoins (DE-613)
- Amides (2) (Comparison of Reversed Phase Columns)
- Amides (3) (DE-413)
- Amides (4) (DE-413)
- Amides (5) (DE-413)
- Aldehydes (DE-413)
- Analysis of Humic Substances by SEC (SB-805 HQ)
- DNPH Derivative Aldehydes (ODP-50 4D)
- Analysis of Pochonicine and Its Analogues in Filamentous Fungi Culture Extract (ES-502C 7C)
- Separation of Furfural and Guaiacol (DE-413)
- Separation of C1 Compounds (DE-413)
- Simultaneous Analysis of Various Nitrogen Compounds According to Japanese Testing Methods for Fertilizers (ES-502C 7C)
- Separation of Substances Produced During Utilization of Biomass (VN-50 4D)
- LC/MS Analysis of Hexamethylenetetramine (VC-50 2D)