In order to separate hydrophilic compounds, water or 100 % buffer solution is used as the eluent, however, such eluent usually shorten the column life of ODS columns. Since the packing material of RSpak DE-413 is chemically stable and has long column life. The stable analysis results can be obtained using DE-413 even when 100 % aqueous solution is used as the eluent.



Sample : 10 μL
1. 200 mg/mL Acetic acid
2. 20 mg/mL Dimethylacetamide

Column       : Shodex RSpak DE-413 (4.6 mm I.D. x 150 mm)
Eluent       : 10 mM Sodium phosphate buffer (pH5.0)
Flow rate    : 1.0 mL/min
Detector     : UV (210 nm)
Column temp. : 40 °C