Three reversed phase columns RSpak DM-614, DE-613 and DS-613 were used for the analysis of alkylamides. For this sample, DM-614 is not suited because of too high polarity of its packing material, and, DS-613 is also not suited because of too long elution time. DE-613 is best suited for this sample.


Sample :
1. Acetamide
2. Propionamide
3. Butylamide
4. Valeramide

Columns      : (A); Shodex RSpak DM-614,
               (B); DE-613,
               (C); DS-613 (6.0 mm I.D. x 150 mm each)
Eluent       : H2O/CH3OH=70/30
Flow rate    : 1.0 mL/min
Detector     : UV (210 nm)
Column temp. : 35 °C