The eluent used here is suitable for the simultaneous analysis of inorganic anions and dibasic and tribasic organic acids using IC I-524A (an anion analysis column for non-suppressor method). Organic acids are contained in beer and they interfere with the quantification of anions. Cl^-, NO₃^- and SO₄^2- can be separated without such interference of organic acids. Organic acids, oxalic acid and citric acid also can be analyzed without interference.


Sample : 10-fold diluted Beer , 100 μL
1. Acetic acid
2. H₂PO₄^- , Succinic acid , Pyroglutamic acid
3. Lactic acid , Pyruvic acid
4. Cl^-
5. Malic acid , Br^-
6. NO₃^-
7. Oxalic acid
8. Citric acid
9. SO₄^2-

Column       : Shodex IC I-524A (4.6 mm I.D. x 100 mm)
Eluent       : 1.5 mM Phthalic acid + 1.38 mM Tris(hydroxymethyl)aminomethane
               + 300 mM Boric acid aq.
Flow rate    : 1.2 mL/min
Detector     : Non-suppressed coductivity
Column temp. : 40 °C