Carbonate was analyzed using IC I-524A, an anion analysis column for non-suppressor method. With this method, standard anions (F-, Cl-, NO2-, Br-, NO3-, HPO42- and SO42-) and carbonate ions are separated. Since presence of Mg2+ and/or Ca2+ may interfere with target analyte peaks, sample pretreatment with Na+ type cation exchange is recommended. When using an alkaline eluent, 10 % methanol should be added to the eluent to prevent the packing material from deteriorating.
Eluent preparation
- 1.Weigh 0.207 g of reagent grade p-hydroxybenzoic acid and add 100 mL of methanol into a volumetric flask and dissolve.
- 2.Add water to make up to 900 mL.
- 3.Add 7.9 mL of 0.2 M reagent grade diethylethanolamine*, followed by additional water to make up to 1000 mL.
- 4.Pass through a 0.2-um membrane filter before use.
*0.2 M diethylethanolamine should be kept in a refrigerator.
Sample: 20 µL
- 1.F- 5 mg/L AsO43- 50 mg/L
- 2.HCO3- 30 mg/L I- 50 mg/L
- 3.Cl- 5 mg/L CrO42- 50 mg/L
- 4.NO2- 5 mg/L S2O32- 50 mg/L
- 5.Br- 10 mg/L WO42- 50 mg/L
- 6.NO3- 10 mg/L MoO42- 50 mg/L
- 7.HPO42- 10 mg/L SCN- 50 mg/L
- 8.SO42- 10 mg/L

- Column
- :Shodex IC I-524A (4.6 mm I.D. x 100 mm)
- Eluent
- :1.5 mM p-Hydroxybenzoic acid + 1.6 mM N,N-Diethylethanolamine + 10 % CH3OH
- Flow rate
- :1.2 mL/min
- Detector
- :Non-suppressed conductivity
- Column temp.
- :40 ℃
Standard anions (F-, Cl-, NO2-, Br-, NO3-, HPO42- and SO42-) as well as carbonate were analyzed using IC I-524A (an anion analysis column for non-suppressor method). When an alkaline eluent is used, peaks of divalent cations (Mg2+, Ca2+) may interfere. In such instances, the sample must be pretreated using an Na+ type cation exchanger and the divalent cations in the sample replaced with Na+.
In case that the pH of the eluent is higher than 7.0, 10 % methanol should be added to maintain the durability of the packing material.
(Preparation of eluent)
1) Put 0.207 g of reagent grade p-hydroxybenzoic acid and 100 mL of methanol into a volumetric flask and dissolve.
2) Add pure water to make up a 900 mL solution.
3) Add 7.9 mL of 0.2 M reagent grade diethylethanolamine.
4) Add purified water to make up a 1 liter solution.
5) Pass through a 0.2 μm membrane filter before use.
(Remark): 0.2 M diethylethanolamine should be kept in a refrigerator.

Sample : 20 μL
1. F- 5 mg/L 2. HCO3- 30 mg/L 3. Cl- 5 mg/L 4. NO2- 5 mg/L 5. Br- 10 mg/L 6. NO3- 10 mg/L 7. HPO42- 10 mg/L 8. SO42- 10 mg/L |
AsO43- 50 mg/L I- 50 mg/L CrO42- 50 mg/L S2O32- 50 mg/L WO42- 50 mg/L MoO42- 50 mg/L SCN- 50 mg/L |
Column : Shodex IC I-524A (4.6 mm I.D. x 100 mm) Eluent : 1.5 mM p-Hydroxybenzoic acid (pH7.8 adjusted with N,N-Diethylethanolamine) + 10 % CH3OH Flow rate : 1.2 mL/min Detector : Non-suppressed coductivity Column temp. : 40 °C
Sample Name Index
- Arsenate, AsO43-
- Bromide, Br-
- Carbonate, CO32-
- Chloride, Cl-
- Chromate, CrO42-
- Fluoride, F-
- Hydrogencarbonate, HCO3-
- Iodide, I-
- Molybdate, MoO42-
- Nitrate, NO3-
- Nitrite, NO2-
- Phosphate dibasic, HPO42-
- Sulfate, SO42-
- Thiocyanate, SCN-
- Thiosulfate, S2O32-
- Tungstate, WO42-
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Anion Standards (1) (I-524A)
- Anion Standards (2) (I-524A)
- Anion Standards (3) (I-524A)
- Anion Standards (6) (I-524A)
- Anion Standards (7) (Anions and Organic Acids) (I-524A)
- Anion Standards (8) (Eluent : Octanesulfonic Acid and Boric Acid) (I-524A)
- Anion Standards (15) (Comparison of NI-424 and I-524A)
- Polyphosphoric Acids (I-524A)
- Effects of pH on Retention Time (1) (I-524A)
- Dynamic Range of Column (I-524A)
- Effects of Sample Load on Column Efficiency (1) (I-524A)
- Tap Water (3) (I-524A)
- Tap Water (4) (I-524A)
- River Water (1) (I-524A)
- High Sensitive Detection Method for Anions (I-524A)
- Anions in Beer (I-524A)
- Chinese Cabbage (I-524A)
- Iodic Ion in Urine (I-524A)
- Milk (I-524A)
- Sea Water (1) (I-524A)
- Sodium Azide (1) (I-524A)
- Lithium Tetrafluoroborate (I-524A)
- Analysis of Hydrophobic Anions (I-524A)