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Effects of Flow Rate on Elution Pattern (KF-802)

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Polystyrene standard (Mw: 500) was analyzed using GPC KF-802, a column for organic SEC (GPC). For improving resolution, the appropriate flow rate should be selected considering the diffusion speed. In this case, a higher resolution can be realized with a lower flow rate. Although a reduction of the flow rate from 2.0mL/min to 1.0mL/min brought about a large difference in resolution, a reduction from 1.0mL/min to 0.5mL/min did not. Considering the time for analysis, the use of 1.0mL/min is recommended for the analysis of the sample.


Sample : PS, Polystyrene 500


Column       : Shodex GPC KF-802 (8.0mmI.D. x 300mm) 
Eluent       : THF
Flow rate    : (A)2.0mL/min
               (B)1.0mL/min
               (C)0.5mL/min
Detector     : UV(254nm)
Column temp. : Room temp.

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