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Effects of Sample Concentration (3) (KF-406LHQ)

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The relationships between the sample concentration and their elution volumes were examined using organic solvent SEC (GPC) semi-micro column, GPC KF-406LHQ. The figure shows increased elution volumes when sample concentrations were increased. Also it shows that this influence is bigger for the larger compounds analysis even at the lower concentration ranges. The table shows recommended maximum sample concentrations and injection volumes based on analytes MW.

Refer to Effects of Sample Concentration (KF-402HQ) and Effects of Sample Concentration (2) (KF-403HQ + KF-402HQ).


Sample : PS , 10μL

Maximum sample concentration and maximum sample load for KF-406

MW range Max.Sample conc.
(%)
Max. Sample load
(μg)
EE1,000
2.0 200
10,000 EE50,000
0.6 60
50,000 EE200,000
0.2 20
200,000 EE500,000
0.1 10
500,000 EE2,000,000
0.05 5
2,000,000 EE5,000,000
0.03 3
5,000,000 EE/TD>
0.02 2
Columns      : Shodex GPC KF-406LHQ (4.6mmI.D. x 250mm) x 2             
Eluent       : THF       
Flow rate    : 0.3mL/min
Column temp. : 40°C

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