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Effects of Temperature (KF-602)


For GPC KF-602, the relaion between HETP (height equivalent to a theoretical plate) and temperature is shown here. In the range of 30 to 50 °C, HETP changes very little. This is also true for other grades of Shodex GPC KF-series columns. When using the columns replacing the in-column solvent from original solvent (THF) to other solvents, check the physical characteristics (viscosity, melting point, boiling point, etc.) of the solvent to be used because the pressure may exceed the maximum usable pressure by vaporization or solidification of a part of the solvent in the column. Usually, the use of KF-series columns in the range of room temperature to 45 °C is recommended.

Sample : 0.1% Dicyclohexylphthalate, 5μL

Column       : Shodex GPC KF-602 (6.0mmI.D. x 150mm)
Eluent       : THF
Flow rate    : 0.5mL/min 
Detector     : UV(254nm)(small cell volume)
Column temp. : 40°C

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