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Effects of Flow Rate (KF-602)

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For GPC KF-602, the relation between HETP (height equivalent to a theoretical plate) and flow rate is shown here. The value of HETP is almost the same in the range of 0.4 to 0.8mL/min. However, outside of the range, the value of HETP is higher. This is true for other grade of Shodex GPC KF-series columns. When using the columns replacing the in-column solvent from original solvent (THF) to other solvents, check the pressure not to exceed the maximum usable pressure because the pressure will be higher if the viscosity of the replaced solvent is higher.
Generally, the use of KF-series columns in the range of 0.4 to 0.6 mL/min is recommended.


Sample : 0.1% Dicyclohexyl phthalate, 5μL


Column       : Shodex GPC KF-602 (6.0mmI.D. x 150mm)
Eluent       : THF 
Detector     : UV(254nm)(small cell volume)
Column temp. : 40°C

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