Three kinds of estrogen (estrone, estradiol and estriol) were separated by Silica C18P 4D, a ODS column.


Sample : 0.1 mg/mL each, 10 μL
1. Estriol
2. 17β-Estradiol
3. Estrone

Column        : Shodex Silica C18P 4D (4.6 mm I.D. x 150 mm)
Eluent        : H2O/CH3CN=65/35
Flow rate     : 1.0 mL/min
Detector      : UV (280 nm)
Column temp.  : 30 °C