Simultaneous Analysis of Water-Soluble Vitamins (DE-413)

Ten kinds of water-soluble vitamins were simultaneously analyzed using RSpak DE-413, a polymer-based reversed phase chromatography column.

Sample: 10 µL

  1. 1.Vitamin B1 200 µM
  2. 2.Vitamin B6 200 µM
  3. 3.Niacinamide 200 µM
  4. 4.Vitamin C 200 µM
  5. 5.Vitamin B3 200 µM
  6. 6.Vitamin B5 200 µM
  7. 7.Vitamin B12 200 µM
  8. 8.Vitamin B2 200 µM
  9. 9.Biotin 800 µM
  10. 10.Folic acid 20 µM
Column
Shodex RSpak DE-G 4A (4.6 mm I.D. x 10 mm) + DE-413 (4.6 mm I.D. x 150 mm)
Eluent
(A); 10 mM H3PO4 aq./(B); CH3CN
High pressure linear gradient;
(B %) 0 % to 20 % (0 to 15 min), 20 % (15 to 16 min), 0 % (16.01 to 25 min)
Flow rate
1.0 mL/min
Detector
PDA (190 - 400 nm)
Column temp.
40 ℃

Sample Name Index

Operation Manual / Certificate of Analysis

Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.

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Applications