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Chiral Separation of Proline (CRX-853)

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A column for chiral separation, ORpak CRX-853, was used to separate D and L optical isomers of proline.


Sample : 10 μL
DL-Proline 100 μg/mL
(in eluent)


Column       : Shodex ORpak CRX-853 (8.0 mm I.D. x 50 mm)
Eluent       : 2 mM CuSO4 aq. 
Flow rate    : 1.0 mL/min
Detector     : UV (240 nm)
Column temp. : 50 °C

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