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Chiral Separation of Asparagine (CRX-853)

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A column for chiral separation, ORpak CRX-853, was used to separate the D and L optical isomers of asparagine.


Sample : 10 μL
50 μg/mL each (in eluent)
1. D-Asparagine 
2. L-Asparagine


Column       : Shodex ORpak CRX-853 (8.0 mm I.D. x 50 mm)
Eluent       : 2 mM CuSO4 aq. 
Flow rate    : 1.0 mL/min
Detector     : UV (230 nm)
Column temp. : 50 °C

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