A column for chiral separation, ORpak CRX-853, was used to separate D and L isomers of aspartic acid.
Sample : DL-Aspartic acid
0.02%, 50μL
1. D-Aspartic acid
2. L-Aspartic acid
Column : Shodex ORpak CRX-853 (8.0mmI.D. x 50mm) Eluent : 8mM CuSO4 aq. Flow rate : 1.0mL/min Detector : UV(230nm) Column temp. : 50°C
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