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Chiral Separation of Mandelic Acid (CRX-853)


A column for chiral separation, ORpak CRX-853, was used to separate D and L isomers of mandelic acid.

Sample : DL-Mandelic acid 0.01%, 20μL
1. D-Mandelic acid
2. L-Mandelic acid

Column       : Shodex ORpak CRX-853 (8.0mmI.D. x 50mm)
Eluent       : 0.25mM CuSO4 aq.
Flow rate    : 1.0mL/min
Detector     : UV(230nm)
Column temp. : 50°C

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