Some metabolites in urine have been added to the health check as special items in Japan recently. From the items, hippuric acid, methylhippuric acid(o-, m-, p-), mandelic acid which are metabolites of toluene, xylene and styrene and creatinine were analyzed using RSpak DE-413 (a column for reversed phase chromatography) within 10 minutes.


Sample : 10 μL
(Left); Standards
1. Creatinine 20 mg/L
2. Mandelic acid 10 mg/L
3. Hippuric acid 10 mg/L
4. o-Methylhippuric acid 10 mg/L
5. p-Methylhippuric acid 10 mg/L
6. m-Methylhippuric acid 10 mg/L

(Right); Urine 100-fold diluted

Column       : Shodex RSpak DE-413 (4.6 mm I.D. x 150 mm)
Eluent       : CH3CN/Buffer=1/5
               (Buffer: 20 mM Sodium phophate + 20 mM β-Cyclodextrin
                + 4.5 mM sodium dodecyl sulfate (pH3.2))
Flow rate    : 1.0 mL/min
Detector     : UV (225 nm)
Colomn temp. : 50 °C