This shows LC/MS analysis of drugs in BSA with a gradient using polymer-based ODP2 HP. Each drug has peak areas between 98 - 107 % (peak areas without BSA are set as 100 %), which means a good separation without receiving ion suppression from protein.


Sample : drugs 500 ng/mL each in BSA (7.0 mg/L), 5 μL
Lidocaine
Chlorpheniramine
Propranolol
Diphenhydramine
Bupivacaine
Verapamil
Imipramine

Column       : Shodex ODP2 HP-2B (2.0 mm I.D. x 50 mm)
Eluent       : (A);10 mM Ammonium acetate aq.
               (B);100 mM Ammonium acetate aq./CH3CN=10/90
               gradient; (A) to (A) to 70 % (B) to 70 % (B) to (A),
                         0 min to 5 min to 10 min to 15 min to 15.01 min
Flow rate    : 0.2 mL/min
Detector     : ESI-MS (SIM Positive)
Column temp. : 40 °C