Reproducibility in LC/MS Analysis (ODP2 HP)

Reproducibility for drug analysis in protein (BSA) was confirmed using polymer-based ODP2 HP. The separation remains good even after 150 runs.


Sample : 500 ng/mL each in 0.7 mg/mL BSA, 5 μL
1. Lidocaine
2. Imipramine


Column       : Shodex ODP2 HP-2B (2.0 mm I.D. x 50 mm)
Eluent       : 10 mM Ammonium acetate aq./CH3CN=70/30
Flow rate    : 0.2 mL/min
Detector     : ESI-MS (TIC positive)
               m/z 235; Lidocaine
               m/z 281; Imipramine
Column temp. : 40 °C

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