LC/MS analysis of drugs in BSA was performed using polymer-based column ODP2 HP. Each drug has peak areas between 83 -100 % (peak areas without BSA are set as 100 %), which means a good separation without receiving ion suppression from protein.


Sample : drugs 500ng/mL each in BSA (7.0 mg/L), 5 μL
Lidocaine
Chlorpheniramine
Propranolol
Diphenhydramine
Bupivacaine
Verapamil
Imipramine

Column       : Shodex ODP2 HP-2B (2.0 mm I.D. x 50 mm)
Eluent       : 10 mM Ammonium acetate aq./CH3CN=70/30
Flow rate    : 0.2 mL/min
Detector     : UV (280 nm), ESI-MS (SIM Positive)
Column temp. : 40 °C