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Analysis of Allantoin (JJ-50 4D)

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Allantoin has anti-inflammatory action and incorporated in eye drops, toothpaste and lotion. In this application, allantoin was analyzed using RSpak JJ-50 4D, a multimode column. JJ-50 4D is packed with gel material with quaternary ammonium groups, and used to analyze highly polar substances using HILIC mode.


Sample : 10µL
1. Allantoin 0.1mg/mL
(in H2O/CH3CN=1/1)


Column       : Shodex RSpak JJ-50 4D (4.6mmI.D. x 150mm)
Eluent       : 10mM Sodium phosphate buffer (pH7.0)/CH3CN=25/75
Flow rate    : 1.0mL/min
Detector     : UV(200nm)
Column temp. : 40°C

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