According to the Japanese Ingredient Standards for Foods for Specified Health Uses (FOSHU), the Quantitative Method for “Polydextrose” specifies the use of a column packed with polystyrene-divinylbenzene cation- and anion-exchange resins (stainless steel column, 7 - 9 mm I.D. and 15 - 30 cm Length) for the determination of glucose, sorbitol, and levoglucosan. The FOSHU calculates polydextrose content by subtracting the amounts of ash, glucose, sorbitol, and levoglucosan (1,6-anhydro-β-D-glucose) from the polydextrose sample. In this application, standard samples of glucose, sorbitol, and levoglucosan were analyzed using SUGAR SH1011, and calibration curves were prepared.
Note: The flow rate was adjusted so that the retention time of glucose was approximately 8.5 minutes.

Sample: 20 µL
Diluted in H₂O

1. 1.Polydextrose 40.0 mg/mL
2. 2.Glucose 2.5 mg/mL
3. 3.Sorbitol 1.6 mg/mL
4. 4.Levoglucosan 2.5 mg/mL