Guar gum is known to suppress blood sugar level raise and reduce cholesterol.
In the United States Pharmacopeia and the National Formulary (USP 39-NF 34*), the ratio of constituting mannose and galactose is defined, and quantification of guar gum should be carried out with a column with L22 packing material and meets following requirements. The SUGAR SP0810 confirmed the requirements were met.

*The version at the time of the application acquisition. 

Sample Solution A : Guar gum dissolved in TFA aq. Mannose and glucose are produced as a result of hydrolysis of galactomannan by acids. The content of galactomannan and ratio of constituting mannose and galactose from the quantitative values of mannose and galactose in the portion of guar gum taken were calculated.
Sample Solution B : Guar gum dissolved in the eluent. No presence of galactose and mannose peaks are observed in the chromatogram of Standard solution B.

Sample: 10 μL

1. System suitability solution:Glucose, Xylose, Galactose, Mannose 5 mg/mL each (in H₂O)
2. Standard solution:Galactose, Mannose 10 mg/mL each (in H₂O)
3. Sample Solution A:Guar gum 25 mg/mL (prepared according to USP method)
4. Sample Solution B:Guar gum 5 mg/mL (in eluent)