The main component of guar gum is galactomannan, composed of mannose and galactose, and it is known to suppress blood sugar level raise and reduce cholesterol. According to the United States Pharmacopeia and the National Formulary (USP 39-NF 34*), "ASSAY: Content of Galactomannan and Ratio of Constituting Mannose and Galactose" for "Guar Gum" should be carried out with a column filled with L22 packing material. The SUGAR SP0810, a column filled with L22 packing material, confirmed the requirement was met.

System suitability requirementsResolution between dextrose and xylose (System suitability solution): ≥ 0.9
Resolution between xylose and galactose (System suitability solution): ≥ 1.0
Resolution between galactose and mannose (System suitability solution): ≥ 1.5
Tailing factor of galactose and mannose peaks (Standard solution): 0.8 - 1.8
Relative standard deviation (RSD) of galactose and mannose peaks (Standard solution): ≥ 2.0%

*The version at the time of the application acquisition.

Sample: 10 µL each
(System suitability solution) 5 mg/mL each of Galactose, Mannose, Xylose and in eluent
(Standard solution) 10 mg/mL of Galactose and Mannose in eluent
(Sample Solution A**) 25 mg/mL of Guar Gum in TFA solution (prepared according to "Sample Solution A")
(Sample Solution B***) 5 mg/mL of Guar Gum in eluent

1. 1.Glucose
2. 2.Xylose
3. 3.Galactose
4. 4.Mannose
5. 5.Guar Gum

**Quantify the mannose and galactose, and calculate the galactomannan content and the ratio of mannose to galactose in guar gum.
***Confirm that guar gum does not contain mannose and galactose.