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Analysis of Guar Gum According to USP-NF Method (SP0810)

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Guar gum is known to suppress blood sugar level raise and reduce cholesterol.
In the United States Pharmacopeia and the National Formulary (USP 39-NF 34*), the ratio of constituting mannose and galactose is defined, and quantification of guar gum should be carried out with a column with L22 packing material and meets following requirements. The SUGAR SP0810 confirmed the requirements were met.

System suitability requirements:
   Resolution of dextrose (= glucose) and xylose (in the system suitability solution): ≥ 0.9
   Resolution of xylose and galctose (in the system suitability solution): ≥ 1.0
   Resolution of galactose and mannose (in the system suitability solution): ≥ 1.5
   Tailing factor of galactose and mannose (in the standard solution): 0.8 – 1.8
   Relative standard deviation (RSD) of galactose and mannose peaks (in the standard solution) : ≤ 2.0%

*The version at the time of the application acquisition.  

Sample Solution A : Guar gum dissolved in TFA aq. Mannose and glucose are produced as a result of hydrolysis of galactomannan by acids. The content of galactomannan and ratio of constituting mannose and galactose from the quantitative values of mannose and galactose in the portion of guar gum taken were calculated.
Sample Solution B : Guar gum dissolved in the eluent. No presence of galactose and mannose peaks are observed in the chromatogram of Standard solution B.


Sample : 10 μL
System suitability solution : Glucose, Xylose, Galactose, Mannose 5 mg/mL each (in H2O)
Standard solution : Galactose, Mannose 10 mg/mL each (in H2O)
Sample Solution A : Guar gum 25 mg/mL (prepared according to USP method)
Sample Solution B : Guar gum 5 mg/mL (in eluent)

Column       : Shodex SUGAR SP0810 (8.0 mm I.D. x 300 mm)
Eluent       : H2O
Flow rate    : 0.75 mL/min
Detector     : Shodex RI
Column temp. : 80 °C

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