Chondroitin sulfate is an acidic mucopolysaccharide which has a chain alternating disaccharide units composed of N-acetyl-D-galactosamine and D-glucuronic acid; it is found  in animal tissues. There are seven kinds of chondroitin sulfate (A, B, C, D, E, H and K) which differ in positions and the number of sulfate groups. Chondroitin sulfate B, which is known as dermatan sulfate, contains the L-iduronic acids in place of D-glucuronic acids as uronic acids. In this application, three kinds of chondroitin sulfate were analyzed using OHpak SB-806M HQ, a column for aqueous SEC column.


Sample : 0.1 % each, 50 μL

Columns      : Shodex OHpak SB-806M HQ (8.0 mm I.D. x 300 mm) x 2
Eluent       : 0.1 M NaNO3 aq.
Flow rate    : 1.0 mL/min
Detector     : RI
Column temp. : 40 °C