Analysis of D-chiro-Inositol (KS-801)

According to the United States Pharmacopeia and the National Formulary (USPNF 2025 Issue 1*), the ASSAY for D-chiro-Inositol is carried out with a column filled with L58 packing material. By using this assay specifications as a reference, analysis was conducted using a sugar analysis column, SUGAR KS-801.

*The version at the time of the application acquisition.

Sample: 6 µL

  1. 1.Sucrose 0.01 %
  2. 2.Pinitol 0.01 %
  3. 3.Dextrose 0.01 %
  4. 4.D-chiro-Inositol 0.1 %
  5. 5.myo-Inositol 0.01 %
Chromatogram of chiro-Inositol
Column
Shodex SUGAR KS-G 6B (6.0 mm I.D. x 50 mm) + KS-801 (8.0 mm I.D. x 300 mm)
Eluent
H2O
Flow rate
0.6 mL/min
Detector
RI (50 ℃)
Column temp.
80 ℃
Autosampler temp.
10 ℃

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