Lactobionic acid is an acidic oligosaccharide composed of galactose bonded to gluconic acid. It is known to promote calcium absorption and support intestinal health. In this application, lactobionic acid was analyzed under HILIC mode using a polymer-based amino column Asahipak NH2P-50 4E. The calibration curve for lactobionic acid showed high linearity over the concentration range of 1 to 10 mg/mL.

Preparation of Standard Solution

1. (1)Dry calcium lactobionate monohydrate at 107 ℃ for 3 hours.
2. (2)After cooling in a desiccator, accurately weigh 1 g, dissolve it in water, and dilute to 100 mL.
3. (3)Dilute a portion of the solution prepared in step (2) with water to prepare standard solutions of 1, 2, 5, and 10 mg/mL for making a calibration curve.

Sample: 10 µL
(Standard solution) Calcium lactobionate monohydrate 5 mg/mL in H₂O

1. 1.Lactobionic acid