Maltobionic acid, a carbohydrate, is an acidic oligosaccharide in which gluconic acid is linked to glucose. It is reported to promote mineral absorption, maintain bone density, and exert intestinal regulatory effects. Maltobionic acid was analyzed in HILIC mode using Asahipak NH2P-50 4E, a polymer-based amino column. A calibration curve with good linearity was obtained in a concentration range of 1 to 4 mg/mL. Analysis of a commercial yogurt drink containing calcium maltobionate as a real sample confirmed that the recovery of calcium maltobionate was close to 100%.

Sample Preparation

1. 1.Accurately pipette 1 mL of a yogurt drink and dilute to 10 mL with water.
2. 2.Take 160 µL of the solution from step 1, add 640 µL of water and mix.
3. 3.Add 200 µL of 1 M NaOH aqueous solution to neutralize.
4. 4.Add 1000 µL of acetonitrile.
5. 5.Filter through a 0.45-µm membrane filter and use it as a sample.

Sample: 10 µL
(Standard solution) Calcium maltobionate 2 mg/mL in CH₃CN/H₂O=50/50
(Sample solution) Pretreated yogurt drink

1. 1.Maltobionic acid