Monosaccharides and Disaccharides (8) (VG-25 4D)

Monosaccharides and disaccharides were analyzed by HILICpak VG-25 4D, a polymer-based amino column. The VG-25 4D column achieves higher resolution than the existing VG-50 4Ecolumn. By using the described method, 6 types of monosaccharides and disaccharides were simultaneously analyzed.

Sample: 5 μL
0.1 % each

  1. 1.Fructose
  2. 2.Galactose
  3. 3.Glucose
  4. 4.Sucrose
  5. 5.Lactose
  6. 6.Maltose
Chromatogram of lactulose
Column
Shodex HILICpak VG-25 4D (4.6 mm I.D. x 150 mm)
Eluent
CH3CN/CH3OH/H2O=75/20/5
Flow rate
0.8 mL/min
Detector
RI
Column temp.
60 ℃

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