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Elution Volume of Saccharides

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Elution volumes of saccharides for seven different columns, SUGAR SP0810, SC1011, KS-801, SZ5532, SC1211, RSpak DC-613, Asahipak NH2P-50 4E and GS-220 HQ are shown in the table. As only the peak top position can be obtained from the table, if you want to to see the chromatogram with actual peak width, please contact us.


Substances
Elution Volume (mL)
SUGAR RSpak Asahipak
SP0810 SC1011 KS-801 SZ5532 SC1211 DC-613 NH2P-50 4E GS-220 HQ x 2
Pb2+ Ca2+ Na+ Zn2+ Ca2+ Na+
N-Acetyl-α
-D-glucosamine
8.86 7.75 6.68 2peaks 4.10 2peaks 6.66  
D(+)-Arabinose 10.42 8.91 8.21 5.11 5.56 5.75 6.18  
D-Arabitol 15.86 11.33 7.63 7.27 8.16 5.81 6.29  
Aspartame - - - - 34.02 - -  
2-Deoxy-D-glucose 8.83 7.58 7.15 4.34 4.02 4.43 6.02  
Difructose anhydride III 7.07 6.30 5.81 4.30 * 4.51 7.77  
Dulcitol 20.18 12.76 7.40 9.46 11.28 7.33 7.45  
meso-Erythritol 12.70 10.09 7.86 5.73 6.27 4.84 5.43 17.38
Ethanol 11.13 11.33 10.09 - 4.27 - -  
1-Fructofuranosyl
-D-nystose
6.05 5.27 4.76 31.43 * - - 14.24
D(-)-Fructose 11.05 8.85 7.71 5.37 5.90 6.19 6.75 17.05
D(+)-Fucose 10.48 8.84 8.09 4.50 4.96 4.81 5.43  
D(+)-Galactose 9.74 7.98 7.58 6.46 4.98 7.28 8.10 16.60
4'-Galactosyllactose 7.42 6.02 5.40 19.02 * 21.24 21.66  
α-D-Galacturonic acid - 6.28 4.36 - 5.63 - -  
Gentiobiose 7.22 6.08 5.75 10.50 * 13.18 16.36  
Glucose 8.63 7.30 7.17 5.87 4.76 6.83 8.61 16.75
Glycerol           4.26   17.83
Glycyrrhizin - - - - 2.71 2.00 -  
myo-Inositol 12.77 8.86 7.99 12.63 7.87 15.80 9.96  
Isomaltose 7.68 6.26 5.95 10.57 * 13.82 15.18 15.57
Isomaltotriose 7.09 5.75 5.34 21.17 * 32.02 27.55 14.72
1-Kestose 6.79 5.75 5.26 13.09 * - 20.11 15.31
Kojibiose 7.56 6.21 5.88 9.65 * 11.47 14.82  
Lactitol 13.27 8.09 6.13 16.35 6.67 14.04 11.82 15.50
Lactose 8.05 6.51 5.99 10.12 4.07 11.69 13.27 15.71
Lactosylfructoside 7.12 5.88 5.29 14.69 * 15.75 18.98  
Lactulose 9.13 6.99 6.19 9.16 4.65 10.80 10.72  
Maltitol 12.23 8.26 6.03 13.04 6.77 11.81 11.82 15.82
Maltoheptaose           -   14.82
Maltohexaose           -   14.56
Maltopentaose           -   14.31
Maltose 7.85 6.34 5.94 8.67 * 10.61 14.24 16.11
Maltotriose 7.48 5.89 5.38 13.79 * 17.88 24.96 15.56
Mannitol 15.80 11.10 7.23 8.75 9.03 6.84 7.39  
D-Mannose 10.72 8.17 7.64 5.83 5.01 6.72 7.84  
D(+)-Melezitose 6.94 5.79 5.24 13.60 * 14.68 19.27  
Melibiose 8.16 6.45 5.98 11.69 4.23 14.83 14.70  
Methyl-α
-D-mannopyranoside
11.13 8.87 7.78 3.99 4.39 4.15 4.71  
Nystose 6.38 5.45 4.93 20.05 * - 31.90 14.72
Palatinit 2peaks 2peaks 5.90 2peaks 2peaks 2peaks 12.73  
Palatinose 7.84 6.45 5.89 8.08 3.99 9.81 12.12  
Panose 7.14 5.78 5.32 16.87 * 23.14 25.60  
L-Phenylalanine - - - - 31.58 - -  
D(+)-Raffinose 7.14 5.78 5.29 16.36 * 19.11 20.25 15.08
D(+)-Rhamnose 9.77 8.23 7.37 3.93 4.43 4.09 5.52  
D(-)-Ribose 19.35 13.66 9.04 4.82 8.64 5.30 5.45  
Rutinose 7.81 6.49 5.80 6.65 * 7.21 10.87  
Saccharin sodium 6.96 5.44 4.33 6.77 * 2.04 5.68  
D(-)-Sorbitol 21.61 13.31 7.42 9.79 11.88 7.27 7.09 16.60
D(+)-Sorbose 9.67 8.03 7.38 5.12 4.92 5.91 7.35  
Stachyose 6.82 5.57 4.97 - * - 36.22 14.33
Stevioside - - - 4.14 * 4.44 6.07  
Sucrose 7.54 6.29 5.87 7.91 * 8.68 11.87 16.26
α-D-Talose 21.33 12.59 8.76 5.69 8.51 6.32 6.47
Theanderose 2peaks 2peaks 2peaks 2peaks *   2peaks  
Trehalose 7.62 6.27 5.78 10.85 * 11.49 13.25  
Trehalulose 8.92 6.95 6.10 9.54 4.78 11.38 11.68  
Xylitol 19.87 13.14 7.94 7.77 10.16 6.19 6.10 16.88
Xylobiose 8.16 6.68 6.40 5.65 * 6.71 9.05  
D(+)-Xylose 9.21 7.90 7.71 4.55 4.48 5.21 6.58 17.46
D-Xylulose 10.64 9.02 8.04 4.06 5.07 4.56 5.41  

(-)-->cannot be detected
(*)-->cannot be separated from solvent peak

 

Columns      : Shodex SUGAR SP0810, SC1011, KS-801 (8.0mmI.D. x 300mm each)
Eluent       : H2O
Flow rate    : 1.0mL/min
Detector     : Shodex RI
Column temp. : 80°C
Column       : Shodex SUGAR SZ5532 (6.0mmI.D. x 150mm)
Eluent       : H2O/CH3CN=25/75
Flow rate    : 1.0mL/min
Detector     : Shodex RI
Column temp. : 60°C
Column       : Shodex SUGAR SC1211 (6.0mmI.D. x 250mm)
Eluent       : H2O/CH3CN=65/35
Flow rate    : 1.0mL/min
Detector     : Shodex RI
Column temp. : 70°C
Column       : Shodex RSpak DC-613 (6.0mmI.D. x 150mm)
Eluent       : H2O/CH3CN=25/75
Flow rate    : 1.0mL/min
Detector     : Shodex RI
Column temp. : 70°C
Column       : Shodex Asahipak NH2P-50 4E (4.6mmI.D. x 250mm)
Eluent       : H2O/CH3CN=25/75
Flow rate    : 1.0mL/min
Detector     : Shodex RI
Column temp. : 30°C
Column       : Shodex Asahipak GS-220 HQ (7.5mmI.D. x 300mm) x 2
Eluent       : H2O
Flow rate    : 0.5mL/min
Detector     : Shodex RI
Column temp. : 60°C

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