tRNA was analyzed using IEC DEAE-825, a column for weak anion exchange chromatography.


Sample : 10 µL
tRNA (from E.Coli) 0.4 %

Column       : Shodex IEC DEAE-825 (8.0 mm I.D. x 75 mm)
Eluent       : (A); 20 mM Sodium phosphate buffer (pH6.6) + 250 mM KCl 
                    + 5 M Urea + 0.5 mM EDTA 
               (B); (A) + 250 mM KCl
               Linear gradient: 0 min to 150 min, (A) to (B)
Flow rate    : 1.0 mL/min
Detector     : UV (260 nm) 
Column temp. : Room temp.