As Asahipak GS-320 HQ is packed with a packing material bonded with trace amounts of ion exchange groups, the multimode, which combines size exclusion mode predominately with ion exchange mode and reversed phase mode, can be used for the separation of nucleotides.


Sample : 20 μg/mL each, 20 μL
1. 2'AMP
2. 3'AMP
3. cAMP

Column       : Shodex Asahipak GS-320 HQ (7.5 mm I.D. x 300 mm)
Eluent       : 200 mM NaH2PO4 aq./200 mM Na2HPO4 aq.=500/15
Flow rate    : 1.0 mL/min
Detector     : UV (260 nm)
Column temp. : 30 °C