Peptides in Extract from Waste Water Sludge

Peptides in extract from waste water sludge were analyzed using Asahipak GS-320 7G (a multimode column).
GS-320 7G is an old type of GS-320 HQ.


Sample : Peptides in extract from waste water sludge
Acetone
Angiotensin Ⅰ
Angiotensin Ⅲ
Dextran
Insulin
Myoglobin
Ovalbumin

Column       : Shodex Asahipak GS-320 7G (7.5 mm I.D. x 500 mm)
Eluent       : [50 mM NH3-NH4Cl (pH10.3) + 300 mM NaCl]/CH3CN=80/20
Flow rate    : 1.0 mL/min
Detector     : UV (280 nm)
Column temp. : 30 °C

Courtesy of Dr. Hirai, Tokyo Institute of Technology