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Comparison of SEC Separation of IgG between LW-403 4D and LW-803


PROTEIN LW-403 4D is an aqueous SEC (GFC) semi-micro column for rapid analysis of antibody proteins. PROTEIN LW-403 4D and PROTEIN LW-803 (a standard column) were compared for the chromatogram of IgG. The analysis time using LW-403 4D can be completed in half the time compared to LW-803.

Sample : IgG from human serum 10 mg/mL
(LW-403 4D) 0.5 μL
(LW-803) 5 μL

(D) Dimer
(M) Monomer

Columns      : Shodex PROTEIN LW-403 4D (4.6 mm I.D. x 150 mm)
               Shodex PROTEIN LW-803 (8.0 mm I.D. x 300 mm)
Eluent       : 50 mM Sodium phosphate buffer (pH7.0) + 0.3 M NaCl
Flow rate    : (LW-403 4D) 0.35 mL/min
               (LW-803)    1.0 mL/min
Detector     : (LW-403 4D) UV (280 nm) (small cell volume)
               (LW-803)    UV (280 nm) (conventional type)
Column temp. : Room temp.

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