Effects of Injection Volume of Serum

Effects of injection volume when analyzing serum using MSpak GF-310 4B (a column for rapid analysis of drugs) was shown.


Sample : Control serum I Wako
(from Wako Pure Chemical Industries, Ltd.)


Column       : Shodex MSpak GF-310 4B (4.6 mm I.D. x 50 mm)
Eluent       : 50 mM CH3COONH4 aq./CH3CN=70/30
Flow rate    : 0.25 mL/min
Detector     : UV (220 nm)
Column temp. : 30 °C