The in-column solvent of CLNpak EV-2000 can be replaced easily without affecting column performance.
The column shipping solvent of EV-2000 column is ethyl acetate/cyclohexane(3/7).
(1) After pumping dichloroethane/cyclohexane(3/7) at 2 mL/min for 2 h.
(2) After pumping acetone/cyclohexane(2/8) at 2 mL/min for 2 h.
(3) After pumping dichloroethane/cyclohexane(3/7) at 2 mL/min for 2 h.
(4) After pumping acetone/cyclohexane(2/8) at 2 mL/min for 2 h.


Sample :
1. Corn oil
2. Fluvalinate
3. Chinomethionate

Columns      : Shodex CLNpak EV-G (20 mm I.D. x 100 mm) + EV-2000 (20 mm I.D. x 300 mm) 
Flow rate    : 4.0 mL/min
Detector     : UV (254 nm)
Column temp. : 25 °C