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Feature of High Temperature Analysis (1) (High Resolution)


ET-RP1 4D is polymer base column for high temperature analysis. Figure shows comparison of reduced plate height and analysis time between 40°C and 150°C. Smaller value of reduced plate height means better column resolution. The reduced plate height of acetophenone and benzene decreased at the 150°C. Mobile phase viscosity decreases at higher temperature and thus enhanced diffusion rate between the eluent and the polymeric stationary phase. Then resolution is improved.

Sample :
1. Uracil
2. Pyridine
3. Acetophenone
4. Benzene
(notes) Eluent was introduced to column after preheating and introduced to detector after cooling.

Column      : Shodex ET-RP1 4D (4.6mmI.D. x 150mm) : Shodex ET-RP1 4D (4.6mmI.D. x 150mm)
Eluent      : H2O/CH3CN=50/50                       : H2O/CH3CN=75/25
Flow rate   : 0.5mL/min                            : 2.4mL/min
Detector    : Diode Alley (210nm)                  : Diode Alley (210nm)
Column temp.: 40°C                                 : 150°C

*Column oven : Polaratherm 9000 Series (SandraSelerity Technologies, Inc)

Coutesy of Research Institude for Chromatography bvba

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