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High Performance Semi-micro Aqueous SEC (GFC) Columns: KW400

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KW400 · Reduced packing material particle size enhances column performance
· Three to four-fold higher sensitivity than KW-800 series
· KW405-4F is applicable analyzing samples with molecular weight above 1,000,000
· Fulfills USP L20, L33, and L59 requirements


(High performance semi-micro columns)

Product codeProduct name*Plate number
(TPN/column)
Particle size
(μm)
Size (mm)
I.D. x Length
Shipping
solvent
F6989201
F6989202
F6989203
F6989204
F6700132
KW402.5-4F
KW403-4F
KW404-4F
KW405-4F
KW400G-4A
≥ 35,000
  ≥ 35,000
  ≥ 25,000
 ≥ 25,000
(guard column)
3
3
5
5
5
4.6 x 300
4.6 x 300
4.6 x 300
4.6 x 300
4.6 x 10
H2O
H2O
H2O
H2O
H2O
Product nameMax. usable
pressure (MPa)
Recommended
flow rate
(mL/min)
Max. usable
flow rate
(mL/min)
Usable temp.
range (°C)
Usable
pH range
Usable salt
conc.
KW402.5-4F
KW403-4F
KW404-4F
KW405-4F
KW400G-4A
10
10
7
7
-
0.35
0.35
0.35
0.35
-
0.5
0.5
0.5
0.5
-
5 to 45
5 to 45
5 to 45
5 to 45
5 to 45
3.0 to 7.5
3.0 to 7.5
3.0 to 7.5
3.0 to 7.5
3.0 to 7.5
≤ 0.3 M
≤ 0.3 M
≤ 0.3 M
≤ 0.3 M
≤ 0.3 M

*Measured with uridine
Usable organic solvent concentration: Up to 100 % for methanol and acetonitrile.
KW400 series is recommended to be used with semi-micro type devices.

[Target molecular weight range and exclusion limit]
Please use the following tables for reference purposes only when selecting columns.

Measured with protein (eluent: phosphate buffer)

Product nameTarget molecular weight rangeExclusion limit
KW402.5
KW403
KW404
KW405
5,000 - 70,000
10,000 - 500,000
30,000 - **(4,000,000)
200,000 - **(20,000,000)

150,000
600,000
**(4,000,000)
**(20,000,000)

**( ) Estimated value

Measured with pullulan (eluent: ultrapure water)

Product nameTarget molecular weight rangeExclusion limit
KW402.5
KW403
KW404
KW405
2,000 - 40,000
3,000 - 50,000
20,000 - 300,000
100,000 - 700,000

60,000
80,000
400,000
1,300,000

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