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Eluent Conditions for PROTEIN KW Series and LW Series


1) Typical eluent
The following solutions are generally used as the eluent.
Phosphate buffer solution
Tris hydrochloric solution
Acetate buffer solution

2) Salt to be added
Salts such as sodium chloride, sodium sulfate, potassium sulfate and ammonium sulfate can be used. Please keep total concentration of salts under 1 M. Their recommended concentration ranges are 0.1 - 0.3 M.

(1) The pH range of the eluent should be 3.0 to 7.5 range.
(2) Use of salts containing chlorine ions should preferably be avoided, as chlorine ions are corrosive to the column or piping of the instrument.

3) Use of urea or guanidlne hydrochloride
Urea or 6M guanidine hydrochloride solutions, which are often used as modifier of proteins, can be used as the eluent.
Since the replacement of the eluent takes a fairly long time, it is recommended to use one column specifically for such an eluent.

4) Use of surfactant
Aqueous solutions of surfactant such as SDS and Brij can also be used.

5) Use of polar organic solvent
Polar organic solvents, such as acetonitrile, methanol and ethanol can also be used as the eluent.

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