Cation Exchange Chromatography Columns

SP-825 CM-825	· Suitable for analyzing of relatively high molecular weight compounds: proteins, peptides, DNA, and RNA · Usable in a wide pH range from pH 2 to 12
SP-FT 4A	· Non-porous base material · Provides ultra-rapid analysis using conventional devices
ES-502C 7C	· Compared to IEC series columns, polyvinyl alcohol is used as base material and this offers different separation pattern · Low hydrophobic interaction with proteins allows analysis under mild conditions
P-421S	· Column for amino acids analysis by cation exchange mode · Supports simultaneous analysis of different amino acids · Fulfills USP L22 and L58 requirements

[Strong cation exchange resin] Functional group: Sulfopropyl
(Standard columns)

Product code	Product name	Ion exchange capacity (meq/g)	Particle size (μm)	Size (mm) I.D. x Length	Shipping solvent
F6118250	IEC SP-825	0.4	8	8.0 x 75	50 mM Na₂SO₄ aq.
F6113100	IEC SP-FT 4A	0.2	2.7	4.6 x 10	20 mM ^*MES buffer (pH5.6)

Product name	Max. usable pressure (MPa)	Max. usable flow rate (mL/min)	Usable temp. range (°C)	Usable pH range	Usable organic solvent conc.	Usable salt conc.
SP-825	2.0	1.5	10 to 50	2 to 12	≤ 20 %	20 mM to 1.0 M
SP-FT 4A	^**20	3.0	5 to 45	2 to 12	≤ 20 %	≤ 1.5 M

Housing Material of SP-FT 4A: PEEK
^*MES: 2-(N-Morpholino)ethanesulfonic acid
^**Total system pressure (system pressure and column pressure) varies depending on the system, solvent composition, temperature, and gradient conditions used. Use the column within the stated total system max pressure.

(Preparative columns) [Preparative columns are made to order.]

Product code	Product name	Particle size (μm)	Size (mm) I.D. x Length	Sandard column
F6548002 F6709604	IEC SP-2025 IEC SP-G 8B	20 20	20.0 x 150 8.0 x 50	SP-825 (guard column)

[Weak cation exchange resin] Functional group: Carboxymethyl
(Standard columns)

Product code	Product name	Ion exchange capacity (meq/g)	Particle size (μm)	Size (mm) I.D. x Length
F6110002	IEC CM-825	0.4	8	8.0 x 75
F7640001	Asahipak ES-502C 7C	0.55	9	7.5 x 100

Product name	Max. usable pressure (MPa)	Max. usable flow rate (mL/min)	Usable temp. range (°C)	Usable pH range	Usable organic solvent conc.	Usable salt conc.
CM-825	2.0	1.5	10 to 50	2 to 12	≤ 20 %	20 mM to 1.0 M
ES-502C 7C	1.2	1.5	10 to 50	2 to 12	≤ 100 %	20 mM to 0.6 M

Product name	Shipping solvent
CM-825	50 mM Na₂SO₄ aq.
ES-502C 7C	0.1 M Sodium phosphate buffer (pH4.4)

(Preparative columns) [Preparative columns are made to order.]

Product code	Product name	Particle size (μm)	Size (mm) I.D. x Length	Sandard column
F6548003 F6709605	IEC CM-2025 IEC CM-G 8B	20 20	20.0 x 150 8.0 x 50	CM-825 (guard column)
F6840003 F6710021	Asahipak ES-502C 20C Asahipak GS-20G 7B	13 20	20.0 x 100 7.5 x 50	ES-502C (guard column)