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Cation Exchange Chromatography Columns

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SP-825
CM-825
· Suitable for analyzing of relatively high molecular weight compounds: proteins, peptides, DNA, and RNA
· Usable in a wide pH range from pH 2 to 12
SP-FT 4A · Non-porous base material
· Provides ultra-rapid analysis using conventional devices
ES-502C 7C · Compared to IEC series columns, polyvinyl alcohol is used as base material and this offers different separation pattern
· Low hydrophobic interaction with proteins allows analysis under mild conditions
P-421S · Column for amino acids analysis by cation exchange mode
· Supports simultaneous analysis of different amino acids
· Fulfills USP L22 and L58 requirements


[Strong cation exchange resin] Functional group: Sulfopropyl
(Standard columns) 

Product
code
Product nameIon exchange
capacity (meq/g)
Particle size
(μm)
Size (mm)
I.D. x Length
Shipping solvent
F6118250
IEC SP-825 0.4 8
8.0 x 75
50 mM Na2SO4 aq.
F6113100
IEC SP-FT 4A 0.2 2.7
4.6 x 10
20 mM *MES buffer (pH5.6)
Product
name
Max. usable
pressure (MPa)
Max. usable
flow rate
(mL/min)
Usable temp.
range (°C)
Usable
pH range
Usable organic
solvent conc.
Usable
salt conc.
SP-825 2.0 1.5 10 to 50 2 to 12 ≤ 20 % 20 mM to 1.0 M
SP-FT 4A **20 3.0 5 to 45 2 to 12 ≤ 20 % ≤ 1.5 M

Housing Material of SP-FT 4A: PEEK
*MES: 2-(N-Morpholino)ethanesulfonic acid
**Total system pressure (system pressure and column pressure) varies depending on the system, solvent composition, temperature, and gradient conditions used. Use the column within the stated total system max pressure.

[Weak cation exchange resin] Functional group: Carboxymethyl
(Standard columns)

Product
code
Product nameIon exchange
capacity (meq/g)
Particle size
(μm)
Size (mm)
I.D. x Length
F6110002
IEC CM-825 0.4 8
8.0 x 75
F7640001
Asahipak ES-502C 7C 0.55 9
7.5 x 100
Product
name
Max. usable
pressure (MPa)
Max. usable
flow rate
(mL/min)
Usable temp.
range (°C)
Usable
pH range
Usable organic
solvent conc.
Usable
salt conc.
CM-825 2.0 1.5 10 to 50 2 to 12 ≤ 20 % 20 mM to 1.0 M
ES-502C 7C 1.2 1.5 10 to 50 2 to 12 ≤ 100 % 20 mM to 0.6 M
Product nameShipping solvent
CM-825
50 mM Na2SO4 aq.
ES-502C 7C
0.1 M Sodium phosphate buffer (pH4.4)

For amino acids analysis
(Standard column)

Product codeProduct namePlate number
(TPN/column)
Functional
group
Particle size
(μm)
Size (mm)
I.D. x Length
F6354211
F6700210
CXpak P-421S
CXpak P-G
≥ 3,500
Guard column
Sulfo (Na+)
Sulfo (Na+)
6
6
4.6 x 150
4.6 x 10
Product
name
Max. usable
pressure (MPa)
Max. usable
flow rate
(mL/min)
Usable temp.
range (°C)
Usable
pH range
Usable organic
solvent conc.
Shipping
solvent
P-421S
P-G
5.0
-
0.7
-
15 to 63
15 to 63
≥ 3
≥ 3
≤ 15 %
≤ 15 %
H2O
H2O

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