Eluent Conditions for Asahipak ES Series

Total salt concentration for Asahipak ES series should generally be 20 to 600 mM. Do not use pure water alone, as it would result in column degradation. Buffer solutions generally used in ion exchange chromatography with the ES series are of the types and concentrations shown in the following tables, Salt solutions such as NaCl, KCl, Na2SO4 and K2SO4 may also be used either indepedently or in combination with these buffer solutions.

Asahipak ES-502C 7CSelect a buffer which has positive charge ion and has a large buffering capacity at the set pH.Scroll
pH range Buffer
3.8 - 4.3 Sodium formate
4.3 Sodium succinate
4.8 - 5.2 Sodium acetate
5.0 - 6.0 Sodium malonate
5.5 - 6.7 MES
6.7 - 7.6 Sodium phosphate
7.6 - 8.2 HEPES
8.2 - 8.7 BICINE
Asahipak ES-502N 7CSelect a buffer which has negative charge ion and has a large buffering capacity at the set pH.Scroll
pH range Buffer
4.8 - 5.0 N-Methylpiperazine HCl
5.0 - 6.0 Piperazine HCl
5.8 - 6.4 Bis Tris HCl
6.4 - 7.3 Bis Tris Propane HCl
7.3 - 7.7 Triethanolamine HCl
7.5 - 8.0 Tris HCl
8.0 - 8.5 N-Methyldiethanolamine HCl
8.4 - 8.8 Diethanolamine HCl
8.5 - 9.0 1,3-Diaminopropane HCl
9.0 - 9.5 Ethanolamine HCl
MES: 2-(N-morpholino)ethanesulfonic acid
HEPES: 4-(2-hydroxymethyl)-1-piperazine ethanesulfonic acid
BICINE: N,N-bis(2-hydroxyethyl)glycine
Tris: tris(hydroxymethyl)aminomethane
Bis Tris : Bis(2-hydroxyethyl)iminotris(hydroxymethyl)methane
Bis Tris propane: 1,3-Bis[tris(hydroxymethyl)methylamino]propane

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