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Eluent Conditions for IEC-series

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・ The standard eluents are buffers and aqueous solutions of salt such as sodium chloride, potassium chloride, sodium sulfate, and potassium sulfate. Salts may also be added to the buffers. The recommended total salt concentration ranges are 20 mM - 1 M.
・ Up to 20 % (v/v) polar organic solvents such as ethylene glycol and 2-propanol can be added.
・ When using a salt gradient method, perform below equilibration steps to obtain repeatable results.
(i) Introduce 10 mL of Solution A (Lower salt concentration)
(ii) Introduce 40 mL of Solution B (Higher salt concentration)
(iii) Introduce more than 15 mL of Solution A

IEC QA-825、DEAE-825
Select a buffer which has positive charge ion and has a large buffering capacity at the set pH.

pH Range

Buffer

4.8 - 5.0

N-Methylpiperazin·HCl

5.0 - 6.0

Piperazine·HCl

5.5 - 7.3

Bis-Tris*1·HCl

6.4 - 7.3

Bis-Tris Propane*2·HCl

7.3 - 7.7

Triethanolamine·HCl

7.5 - 8.0

Tris·HCl

8.0 - 8.5

N-Methyldiethanolamine·HCl

8.4 - 8.8

Diethanolamine·HCl

8.5 - 9.0

1,3-Diaminopropane·HCl

9.0 - 9.5

Ethanolamine·HCl

*1 2-bis(2-hydroxyethyl amino-2-(hyroxymethyl)-13-propanediol)
*2 1,3-bis(tris(hydroxymethyl)methylamino)propane

IEC SP-825、CM-825
Select a buffer which has negative charge ion and has a large buffering capacity at the set pH.

pH Range

Buffer

3.8 - 4.3

Sodium Formate

4.3 - 4.8

Sodium Succinate

4.8 - 5.2

Sodium Acetate

5.0 - 6.0

Sodium Malonate

5.5 - 7.0

2-(N-morpholino)ethanesulfonic acid (MES)

6.7 - 7.6

Sodium Phosphate

6.8 - 8.2

2-[4-(2-Hydroxyethyl)-1-piperazinyl]-ethanesulfonic acid (HEPES)

7.7 - 9.1

N,N-Bis(2-hydroxyethyl) glycine (BICINE)

8.6 - 10.0

2-(cyclohexylamino)ethanesulfonic acid (CHES)

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