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Titration Curve of IEC DEAE-825


IEC DEAE-825 and Asahipak ES-502N are packed with weak anion exchange resins bonded with diethylaminoethyl groups. The pKa of diethylaminoethyl groups is approximately 7.8, therefore DEAE-825 and ES-502N are generally recommended for the separation of acidic proteins and used most commonly for ion exchange chromatography of proteins.

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