When testing the repeatability of Asahipak ODP-50 4E, a polymer-based column for reversed phase chromatography, under alkaline condition, azithromycin was injected repeatedly according toUSP 39 method. When theoretical plate number for azithromycin and resolution of azithromycin/azaerythromycin A regarding number of injections were measured, theoretical plate number and resolution were not changed after 3,000 injections. It was confirmed that ODP-50 4E is very alkaline-resistant.
Sample : 0.5 mg/mL each, 10 µL
1 Azaerythromycin A
2 Azithromycin
dissolved in 6.7 g/L Dibasic potassium phosphate aq. (pH8.0 adjusted with phosphoric acid)/CH3CN=40/60
Column : Shodex Asahipak ODP-50 4E (4.6 mm I.D. x 250 mm) Eluent : 6.7 g/L Dibasic potassium phosphate aq. (pH11.0 adjusted with 10 M KOH) /CH3CN=40/60 Flow rate : 1.0 mL/min Detector : UV (210 nm) Column temp. : 40 °C
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Operation Manual / Certificate of Analysis
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Applications
- Polymer-based Columns for Reversed Phase Chromatography (ODP2 HP)
- Polymer-based Columns for Reversed Phase Chromatography (Asahipak)
- Polymer-based Columns for Reversed Phase Chromatography (RSpak)
- Polymer-based Columns for Hydrophilic Interaction Chromatography (HILIC) (HILICpak)
- Polymer-based Columns for Hydrophilic Interaction Chromatography (HILIC) (Asahipak)
- Silica-based Reversed Phase Chromatography Columns (ODS Columns)
- Silica-based Reversed Phase Chromatography Columns (ODS Columns for UHPLC)