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Eluent Conditions for RSpak Series

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1. General information
The following factors influence the separation for RSpak D-series columns:
  (a) Composition of the eluent
  (b) pH of the eluent
  (c) Concentration of salt
  (d) Column temperature
Polymer based columns can be used with alkaline conditions. The usable pH range for each column is mentioned in the page of the column specification.
If the protein is contained in the sample, please remove it from the sample solution by pretreatment.

2. DS-613 and 413
(DS-613)
(1) The standard eluent is a mixture of water and acetonitrile or water and methanol. The eluent cannot be 100 % water. Be sure to mix 10 % (v/v) or more for methanol or acetonitrile. Methanol or acetonitrile can be used up to 100 %.
(2) Up to 50 % (v/v) THF is usable.
(3) Buffers such as phosphate, acetate, tris-HCl and aqueous solutions of salts such as sodium chloride, potassium chloride, sodium sulfate can be used up to 0.5 M. The eluent must be in pH range of 2 to 12.
(DS-413)
(1) The eluent cannot be 100 % water. Be sure to mix with organic solvent. Mix 30 % or more for methanol, 10 % or more for acetonitrile, and 5 % or more for THF. The mentioned organic solvents can be used up to 100 %.
(2) Various buffer and aqueous salt solutions can be used. They include buffers such as phosphate, acetate, and Tris and salts such as sodium chloride, potassium chloride and sodium sulfate. Please keep total concentration of salts under 0.5 M. The eluent must be in pH range of 1 to 13.

3. DE-613, 413 and 213
(DE-613)

(1) The standard eluent is a mixture of water and acetonitrile, methanol or ethanol of any ratio.
(2) Buffers such as phosphate, acetate, tris-HCl and aqueous solutions of salts such as sodium chloride, potassium chloride, sodium sulfate can be used up to 0.5 M. The eluent must be in pH range of 2 to 12.
(DE-413, DE-213)
(1) The standard eluent is a mixture of water or buffers, and acetonitrile or methanol of any ratio.
(2) Ethanol, 2-propanol (IPA) and tetrahydrofuran (THF) are usable.
(3) Buffers such as phosphate, acetate, tris-HCl and aqueous solutions of salts such as sodium chloride, potassium chloride, sodium sulfate can be used up to 0.5 M. The eluent must be in pH range of 2 to 12.

4. DM-614
(1) The standard eluent is a mixture of water, buffers or aqueous solutions of different salts, and organic solvents of any ratio.
(2) A mixture of water and acetonitrile, methanol or ethanol of any ratio is usable.
(3) Buffers such as phosphate, acetate, tris-HCl and aqueous solutions of salts such as sodium chloride, potassium chloride, sodium sulfate can be used up to 0.5 M. The eluent must be in pH range of 2 to 10.

5. NN-814
(1) The standard eluent is a buffer solution or a mixture of buffer and polar organic solvent. Buffers include phosphate, acetate, and Tris-HCl; and polar organic solvents include acetonitrile, methanol, and ethanol.
(2) Up to 50 % (v/v) polar organic solvents are usable.
(3) Salts such as sodium chloride, potassium chloride, sodium sulfate can be added up to 0.5 M. The eluent must be in pH range of 2 to 12.

6. JJ-50 2D
(1) A mixture of water and acetonitrile or water and methanol of any ratio can be used. Use of organic solvents other than acetonitrile or methanol is not warrantied.
(2) Various buffer and aqueous salt solutions can be used instead of water. They include buffers such as phosphate, acetate, and Tris and salts such as sodium chloride, potassium chloride, and sodium sulfate. Please keep total concentration of salts under 0.1 M. The eluent must be in pH range of 2 to 10.

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