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Features of RSpak DE Series (2) (Adsorption of Metal Coordination Compound)

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Since hinokititol and oxin-Cu are strong metal coordination compounds, they are adsorbed with the impurity metals in silica gel and the adsorption causes peak tailing. In case of RSpak DE series (polymer-based columns for reversed phase chromatography), the column is free from the effect of metals and sample will not be adsorbed by the column.


Sample : 5μL
(Left)
1. 0.01% Hinokitiol
2. 0.001% Benzene
(Right)
1. 0.01% Oxine-Cu


 

Column       : Shodex RSpak DE-413 (4.6mmI.D. x 150mm)
Eluent       : (Left); (1mM EDTA + 20mM KH2PO4) aq./CH3CN=60/40
               (Right); 20mM H3PO4 aq./CH3CN=95/5
Flow rate    : 1.0mL/min
Detector     : UV(254nm)
Column temp. : 40°C

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