A new member of the VG series, VG-25 4D, is packed with 2.5 µm packing material, smaller than the packing material used in the existing product, VG-50 4D. VG-25 4D showed significantly higher theoretical plate number on all four sugars analyzed over VG-50 4D.
VG-25 4D also exhibited higher separation ability over other manufacturer’s amino column packed with 3 µm particles.
Sample: 5 µL
5 mg/L each
- 1.Fructose
- 2.Mannose
- 3.Glucose
- 4.Sucrose
Theoretical Plate Number of Four Sugars
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| VG-25 4D | VG-50 4D | Silica-based amino column | |
|---|---|---|---|
| Fructose | 16,900 | 6,700 | 13,800 |
| Mannose | 18,500 | 7,900 | 13,900 |
| Glucose | 11,600 | 3,700 | 5,200 |
| Sucrose | 19,500 | 7,800 | 16,600 |
- Column
- ：Shodex HILICpak VG-25 4D, VG-50 4D, Silica-based amino column from other manufacturer (4.6 mm I.D. x 150 mm each)
- Eluent
- ：H2O/CH3CN=20/80
- Flow rate
- ：0.6 mL/min
- Detector
- ：RI
- Column temp.
- ：40 ℃
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.